Identification of DNA-binding of tumor suppressor genes by chromatin immunoprecipitation.

PART I. UNDERSTANDING THE FUNCTION AND REGULATION OF TUMOR SUPPRESSOR GENES 1 Utilizing NMR to Study the Structure of Growth-Inhibitory Proteins Francesca M. Marassi ........................................................................................ 3 2 Generation and Application of Phospho-Specific Antibodies for p53 and pRB Yoichi Taya, Kiichiro Nakajima, Kumiko Yoshizawa-Kumagaye, and Katsuyuki Tamai ................................................................................... 17 3 Stability and Ubiquitination of the Tumor Suppressor Protein p53 Lesley J. Jardine and Carl G. Maki ................................................................. 27 4 Role of Tumor Suppressors in DNA Damage Response Bin-Bing S. Zhou, Michael R. Mattern, and Kum Kum Khanna .................. 39 5 In Situ Hybridization in Cancer and Normal Tissue Shrihari Kadkol, Jeremy Juang, and Tzyy-choou Wu ................................. 51 6 Genetic Strategies in Saccharomyces cerevisiae to Study Human Tumor Suppressor Genes Takahiko Kobayashi, Ting Wang, Hua Qian, and Rainer K. Brachmann ........................................................................... 73 7 Electrophoretic Mobility Shift Analysis of the DNA Binding of Tumor Suppressor Gene Products Edward C. Thornborrow, Matthew Maurer, and James J. Manfredi ........... 87 8 Analysis of Gene Promoter Regulation by Tumor Suppressor Genes Kumaravel Somasundaram, Sanjeev Das, Smita Lakhotia, and Narendra Wajapeyee .......................................................................... 101 9 DNA Footprinting M. Stacey Ricci and Wafik S. El-Deiry .......................................................... 117 10 Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation Timothy K. MacLachlan and Wafik S. El-Deiry ........................................... 129 11 Co-immunoprecipitation of Tumor Suppressor Protein-Interacting Proteins Li-Kuo Su ......................................................................................................... 135